Dataset: Fluorescence spectra from niskin bottle samples collected with depth profiles during R/V Hugh R. Sharp cruise HRS1608 Mid-Atlantic Bight in 2016

Samples were transferred from Niskin bottles to clean 10L low-density polyethylene cubitainers using silicon tubing. Containers were rinsed three times with sample before final collection.  

Subsamples for fluorescence and absorbance were 0.2 µm filtered using Whatman GD/X cellulose acetate syringe filters. Filters were rinsed with ~20mL sample before collecting samples in combusted 40mL amber glass vials. Samples stored at 4°C until analysis (within 1 week of collection).  See "Related Datasets" section for the absorbance data. For fluorescence and absorbance measurements, samples were transferred to 1 cm quartz fluorescence cuvette and analyzed using a Horiba Aqualog spectrofluorometer. Absorbance was recorded from excitation wavelengths 240 to 600 at 3 nm intervals. Fluorescence emission was recorded from ~243 to 297 nm at fixed ~3.3 nm intervals to created excitation-emission matrix (EEM) spectra. Integration time = 2s. Ultrapure water used as the fluorescence blank and was subtracted from all EEM spectra.