Morphometric data on Neocalanus flemingeri females were made on individuals collected in diapause in June and September, 2019 and after incubation in the laboratory for up to 4.5 weeks. Collections were made from depth in Prince William Sound in the Gulf of Alaska during two NGA LTER cruises. After sorting, females were incubated in flasks at 4-6ºC for up to 4.5 weeks. Individuals were removed for imaging and experimental incubations to study oocyte production. Morphometric analysis was used to ...
Show moreSample collection and sorting
Copepods were collected in Prince William Sound, Alaska in the summer and fall of 2019 during the Northern Gulf of Alaska Long Term Ecological Research (NGA LTER) cruises (https://nga.lternet.edu/). Females “PWS2/June” were collected on June 30th, 2019 at the sampling site PWS2 (Latitude 60° 32.1’N; Longitude 147° 48.2’W) (R/V Sikuliaq, cruise number: SKQ201915S), and the “Pleiades/September” females were collected on September 12th and 13th, 2019 at PWS2 and near the Pleiades Islands (Latitude 60° 16.7’N; Longitude 147° 59.2’W) (M/V Tiglax, cruise number: TGX201909). Copepods were collected with a Midi MultiNet (0.25 m2 mouth area; 150 μm mesh nets) towed vertically from near the bottom to the 163 surface at 0.5 m/sec (PWS2: 798 m; KIP2: 588 m). Upon retrieval, net samples were immediately diluted using filtered seawater collected from depth and kept between 4-6°C to minimize thermal stress. All females selected for the experiments were sorted under a dissecting microscope. Females were placed in groups of three into 750 mL Falcon tissue-culture flasks and incubated under dim light in an incubator for up to 4.5 weeks. Experimental emperatures were at or below deep-water temperatures in Prince William Sound (temperature settings: 4°C for June and 6°C for September). A subset of females was used in the DNA replication experiments; the remaining females were imaged for measurements of prosome length and lipid sac area (see the related dataset https://www.bco-dmo.org/dataset/908514).
Experimental design
For the timeline, sets of females were removed from the incubation flasks for imaging and/or incubations in low concentrations of 5-Ethynyl-2’-deoxyuridine (EdU). Individuals that were imaged as well as incubated in EdU are identified in the dataset and provide identifications used in the related dataset.
Lenz, P. H., Hartline, D. K., Monell, K. J. (2024) Post-diapause Neocalanus flemingeri females morphometric measurements and calculations of lipid fullness and lipid volume taken from the R/V Sikuliaq and the R/V Tiglax in the Northern Gulf of Alaska from 2019-06-30 to 2019-09-13. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2024-06-12 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.907880.1 [access date]
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