Dataset: A study using amplicon sequencing of the viral mcp gene of dinoRNAVs to analyze their dynamics in the reef-buliding coral <i>Porites c.f. lobata</i> at three reef zones around Moorea, French Polynesia

Name: A study using amplicon sequencing of the viral mcp gene of dinoRNAVs to analyze their dynamics in the reef-buliding coral Porites c.f. lobata at three reef zones around Moorea, French Polynesia
Published: 2023-09-27
Keywords: dinoflagellate-infection RNA virus, dinoRNAV, coral reefs, heat stress, Porites lobata, Symbiodiniaceae, Cladocopium, temperature, colony health, oceans

Final no updates expectedDOI: 10.26008/1912/bco-dmo.906617.1Version 1 (2023-09-27)Dataset Type:Other Field Results

Principal Investigator: Adrienne M.S. Correa (Rice University)

Principal Investigator: Rebecca Vega Thurber (Oregon State University)

Co-Principal Investigator: Andrew Thurber (Oregon State University)

Scientist: Lauren I. Howe-Kerr (Rice University)

BCO-DMO Data Manager: Shannon Rauch (Woods Hole Oceanographic Institution)

Project: Collaborative Research: Viral Reefscapes: The Role of Viruses in Coral Reef Health, Disease, and Biogeochemical Cycling (Moorea Virus Project)

Abstract

These data are from a study that used amplicon sequencing of the viral major capsid protein (mcp) gene of positive-sense single-stranded RNA viruses known to infect symbiotic dinoflagellates ('dinoRNAVs') to analyze their dynamics in the reef-building coral, Porites lobata. The investigators repeatedly sampled 54 colonies across three environmentally distinct reef zones (fringing reef, back reef, and forereef) around the island of Moorea, French Polynesia over a three-year period and spanning a ...

Cite Dataset

Spatial Extent: N:-17.47 E:-149.80 S:-17.51 W:-149.85
Temporal Extent: 2017-09-12 - 2020-10-28

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This dataset is comprised of three components:

(1) temperature data measured at each reef site - available in the csv data file "906617_v1_temperature_moorea_reefs.csv"

(2) images of Porites lobata colonies taken at each site - available in the PDF "POR colony health images.pdf"

(3) dinoRNAV mcp gene amplicon libraries and Symbiodiniaceae LSU gene amplicon libraries - available in the National Center for Biotechnology Information Sequence Read Archive under accession numbers PRJNA928208 and PRJNA930706, respectively. Parameters used to analyze the viral data are described in Zenodo at https://zenodo.org/record/7552892#.Y_PAa-zMK3I (doi: 10.5281/zenodo.7552892)

Results of this study have been published in Howe-Kerr et al., 2023. (doi: 10.1038/s43705-023-00227-7).

Related Datasets

Rice University. Symbiodiniaceae, Porites lobata Symbiodiniaceae LSU amplicon sequencing 2018-2020 Moorea, French Polynesia. 2023/02. In: BioProject [Internet]. Bethesda, MD: National Library of Medicine (US), National Center for Biotechnology Information; 2011-. Available from: http://www.ncbi.nlm.nih.gov/bioproject/PRJNA930706. NCBI:BioProject: PRJNA930706.

Rice University. dinoRNA virus, Porites lobata Symbiodiniaceae infecting RNA viruses. 2023/01. In: BioProject [Internet]. Bethesda, MD: National Library of Medicine (US), National Center for Biotechnology Information; 2011-. Available from: http://www.ncbi.nlm.nih.gov/bioproject/PRJNA928208. NCBI:BioProject: PRJNA928208.

Related Publications

Results

Howe-Kerr, L. I., Grupstra, C. G. B., Rabbitt, K. M., Conetta, D., Coy, S. R., Klinges, J. G., Maher, R. L., McConnell, K. M., Meiling, S. S., Messyasz, A., Schmeltzer, E. R., Seabrook, S., Sims, J. A., Veglia, A. J., Thurber, A. R., Thurber, R. L. V., & Correa, A. M. S. (2023). Viruses of a key coral symbiont exhibit temperature-driven productivity across a reefscape. ISME Communications, 3(1). https://doi.org/10.1038/s43705-023-00227-7

Methods

Frøslev, T. G., Kjøller, R., Bruun, H. H., Ejrnæs, R., Brunbjerg, A. K., Pietroni, C., & Hansen, A. J. (2017). Algorithm for post-clustering curation of DNA amplicon data yields reliable biodiversity estimates. Nature Communications, 8(1). https://doi.org/10.1038/s41467-017-01312-x

Methods

Grupstra, C. G. B., Howe-Kerr, L. I., Veglia, A. J., Bryant, R. L., Coy, S. R., Blackwelder, P. L., & Correa, A. M. S. (2022). Thermal stress triggers productive viral infection of a key coral reef symbiont. The ISME Journal, 16(5), 1430–1441. https://doi.org/10.1038/s41396-022-01194-y

Methods

Howe-Kerr, L., Veglia, A., &amp; Correa, A. (2023). vAMPirus Analysis - Viruses of a key coral symbiont exhibit temperature-driven productivity across a reefscape (Version 1.0.0). Zenodo. https://doi.org/10.5281/ZENODO.7552892

Methods

Montalvo-Proaño, J., Buerger, P., Weynberg, K. D., & van Oppen, M. J. H. (2017). A PCR-Based Assay Targeting the Major Capsid Protein Gene of a Dinorna-Like ssRNA Virus That Infects Coral Photosymbionts. Frontiers in Microbiology, 8. https://doi.org/10.3389/fmicb.2017.01665

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Dataset: A study using amplicon sequencing of the viral mcp gene of dinoRNAVs to analyze their dynamics in the reef-buliding coral <i>Porites c.f. lobata</i> at three reef zones around Moorea, French Polynesia

Final no updates expectedDOI: 10.26008/1912/bco-dmo.906617.1Version 1 (2023-09-27)Dataset Type:Other Field Results

Principal Investigator: Adrienne M.S. Correa (Rice University)

Principal Investigator: Rebecca Vega Thurber (Oregon State University)

Co-Principal Investigator: Andrew Thurber (Oregon State University)

Scientist: Lauren I. Howe-Kerr (Rice University)

BCO-DMO Data Manager: Shannon Rauch (Woods Hole Oceanographic Institution)

Project: Collaborative Research: Viral Reefscapes: The Role of Viruses in Coral Reef Health, Disease, and Biogeochemical Cycling (Moorea Virus Project)

Abstract

Cite Dataset

Spatial Extent: N:-17.47 E:-149.80 S:-17.51 W:-149.85
Temporal Extent: 2017-09-12 - 2020-10-28

XXX

Views

XX

Downloads

X

Citations

This dataset is comprised of three components:

(1) temperature data measured at each reef site - available in the csv data file "906617_v1_temperature_moorea_reefs.csv"

(2) images of Porites lobata colonies taken at each site - available in the PDF "POR colony health images.pdf"

Results of this study have been published in Howe-Kerr et al., 2023. (doi: 10.1038/s43705-023-00227-7).

Related Datasets

Related Publications

Results

Methods

File(s)	Type	Description	Action
906617_v1_temperature_moorea_reefs.csv (7.57 MB)	Comma Separated Values (.csv)	Water temperature data associated with dataset ID 906617, version 1.	Add to Cart Download

Supplemental File(s)	Type	Description	Action
POR colony health images.pdf (26.67 MB)	Portable Document Format (.pdf)	Images associated with dataset 906617, version 1. This PDF file contains images of focal Porites lobata colonies, taken at each sampling point, and used to determine whether colonies experienced partial mortality during the study duration.	Add to Cart Download

File(s)	Type	Description	Action
906617_v1_temperature_moorea_reefs.csv (7.57 MB)	Comma Separated Values (.csv)	Water temperature data associated with dataset ID 906617, version 1.	Add to Cart Download

Supplemental File(s)	Type	Description	Action
POR colony health images.pdf (26.67 MB)	Portable Document Format (.pdf)	Images associated with dataset 906617, version 1. This PDF file contains images of focal Porites lobata colonies, taken at each sampling point, and used to determine whether colonies experienced partial mortality during the study duration.	Add to Cart Download

Dataset: A study using amplicon sequencing of the viral mcp gene of dinoRNAVs to analyze their dynamics in the reef-buliding coral <i>Porites c.f. lobata</i> at three reef zones around Moorea, French Polynesia

Principal Investigator: Adrienne M.S. Correa (Rice University)

Principal Investigator: Rebecca Vega Thurber (Oregon State University)

Co-Principal Investigator: Andrew Thurber (Oregon State University)

Scientist: Lauren I. Howe-Kerr (Rice University)

BCO-DMO Data Manager: Shannon Rauch (Woods Hole Oceanographic Institution)

Project: Collaborative Research: Viral Reefscapes: The Role of Viruses in Coral Reef Health, Disease, and Biogeochemical Cycling (Moorea Virus Project)

Abstract

Metadata

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XX

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Related Datasets

Related Publications

Dataset: A study using amplicon sequencing of the viral mcp gene of dinoRNAVs to analyze their dynamics in the reef-buliding coral <i>Porites c.f. lobata</i> at three reef zones around Moorea, French Polynesia

Principal Investigator: Adrienne M.S. Correa (Rice University)

Principal Investigator: Rebecca Vega Thurber (Oregon State University)

Co-Principal Investigator: Andrew Thurber (Oregon State University)

Scientist: Lauren I. Howe-Kerr (Rice University)

BCO-DMO Data Manager: Shannon Rauch (Woods Hole Oceanographic Institution)

Project: Collaborative Research: Viral Reefscapes: The Role of Viruses in Coral Reef Health, Disease, and Biogeochemical Cycling (Moorea Virus Project)

Abstract

Metadata

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XX

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Related Datasets

Related Publications