This record contains nucleotide sequences of CsRV1 virus amplified from blue crabs (Callinectes sapidus) across the study region. RNA from crabs identified as infected using a sensitive Rt-qPCR assay was amplified using primers specific to segment and segment 9 of the virus genome. A subset of CsRV1-positive RNA preparations were amplified using a full-genome amplification set of primers to amplify the entire CsRV1 genome. (http://primal.zibraproject.org; Quick et al. 2017). The segment...
Show moreLocation:
Coastal waters and estuaries N. America and S. America, including the Caribbean and Gulf of Mexico. Collections from trapping, dredging, and netting by commercial and recreational fishers, and scientific collections by resource managers.
Methods & Sampling:
Crabs were obtained by collaborating partners in the Caribbean and South America using mixed methods of baited traps and line, nets, and dredges.
DNA sequencing was conducted using both Sanger sequencing (Big Dye terminator) of PCR amplicons at the BioAnalytical Lab in IMET (https://www.umces.edu/baslab).
Illumina-based sequencing of full virus genome amplifications used Illumina MiSeq platform at GENEWIZ (South Plainfield, NJ, USA) with a MiSeq Reagent kit v3 (Illumina, San Diego, CA, USA).
Instruments:
No lab-specific environmental equipment was used to record metadata at the time of crab collection.
For sequence analysis, equipment was 3130 XL Genetic Analyzer from Life Technologies at IMET and contracted Illumina MiSeq platform at GENEWIZ (South Plainfield, NJ, USA) with a MiSeq Reagent kit v3 (Illumina, San Diego, CA, USA).
Taxonomic identifiers:
blue crabs, Callinectes sapidus, urn:lsid:marinespecies.org:taxname:107379
Schott, E., Plough, L. V., Zhao, M. (2023) Nucleotide sequences of CsRV1 virus amplified from blue crabs (Callinectes sapidus) from coastal waters and estuaries in North and South America, including the Caribbean and Gulf of Mexico from 2006 to 2020. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2023-02-28 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.890835.1 [access date]
Terms of Use
This dataset is licensed under Creative Commons Attribution 4.0.
If you wish to use this dataset, it is highly recommended that you contact the original principal investigators (PI). Should the relevant PI be unavailable, please contact BCO-DMO (info@bco-dmo.org) for additional guidance. For general guidance please see the BCO-DMO Terms of Use document.