Dataset: Nucleotide sequences of CsRV1 virus amplified from blue crabs (Callinectes sapidus) from coastal waters and estuaries in North and South America, including the Caribbean and Gulf of Mexico from 2006 to 2020

Location: 
Coastal waters and estuaries N. America and S. America, including the Caribbean and Gulf of Mexico. Collections from trapping, dredging, and netting by commercial and recreational fishers, and scientific collections by resource managers.

Methods & Sampling:
Crabs were obtained by collaborating partners in the Caribbean and South America using mixed methods of baited traps and line, nets, and dredges.

DNA sequencing was conducted using both Sanger sequencing (Big Dye terminator) of PCR amplicons at the BioAnalytical Lab in IMET (https://www.umces.edu/baslab).

Illumina-based sequencing of full virus genome amplifications used Illumina MiSeq platform at GENEWIZ (South Plainfield, NJ, USA) with a MiSeq Reagent kit v3 (Illumina, San Diego, CA, USA).

Instruments:
No lab-specific environmental equipment was used to record metadata at the time of crab collection.

For sequence analysis, equipment was 3130 XL Genetic Analyzer from Life Technologies at IMET and contracted  Illumina MiSeq platform at GENEWIZ (South Plainfield, NJ, USA) with a MiSeq Reagent kit v3 (Illumina, San Diego, CA, USA).

Taxonomic identifiers:
blue crabs, Callinectes sapidus, urn:lsid:marinespecies.org:taxname:107379