Dataset: Pigment concentrations derived from High-Performance Liquid Chromatography (HPLC) analysis from samples collected during the Tara Pacific expedition from 2016-2018

Final no updates expectedDOI: 10.26008/1912/bco-dmo.889930.1Version 1 (2023-02-24)Dataset Type:Cruise Results

Principal Investigator: Lee Karp-Boss (University of Maine)

Student: Guillaume Bourdin (Tara Ocean Foundation)

BCO-DMO Data Manager: Shannon Rauch (Woods Hole Oceanographic Institution)

Project: Island mass effects on planktonic communities in the open ocean (Island Mass Effect)

Abstract

The Tara Pacific expedition (2016-2018) sampled coral ecosystems around 32 islands in the Pacific Ocean. Here we provide pigment concentration data originating from 545 stations that were sampled during the expedition in the Pacific and during transit across the Atlantic. Pigment concentrations were derived from High-Performance Liquid Chromatography (HPLC) analysis. This data set provides high-quality measurements of major pigments including chlorophylls a, b, and c, peridinin, 19'-butanoyloxyf...

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Water samples (volume: 5 liters) for High-Performance Liquid Chromatography (HPLC) analysis were collected from surface water using a custom-made, underway pumping system (nicknamed "Dolphin"; Lombard et al., 2022) and filtered onto 25 millimeter (mm) diameter, 0.7 micrometer (um) pore glass fiber filters. Filters were flash frozen and stored in liquid nitrogen until arrival to the Laboratoire d'Oceanographie de Villefranche (LOV) where they were stored in -80 degrees Celsius (C) until analysis. Samples were analyzed as described in Ras et al., 2008. Pigment extraction was carried out in 3 milliliters (mL) Methanol containing an internal standard (Vitamin E acetate, Sigma). Methanol and Vitamin E acetate were injected regularly during each run to check for retention time reproducibility, peak area precision (<1%), and instrument stability.

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Methods
Lombard, F., Bourdin, G., Pesant, S., Agostini, S., Baudena, A., Boissin, E., Cassar, N., Clampitt, M., Conan, P., Silva, O. D., Dimier, C., Douville, E., Elineau, A., Fin, J., Flores, J. M., Ghiglione, J. F., Hume, B. C. C., Jalabert, L., John, S. G., … Gorsky, G. (2022). Open science resources from the Tara Pacific expedition across coral reef and surface ocean ecosystems. https://doi.org/10.1101/2022.05.25.493210
Methods
Ras, J., Claustre, H., &amp; Uitz, J. (2008). Spatial variability of phytoplankton pigment distributions in the Subtropical South Pacific Ocean: comparison between in situ and predicted data. Biogeosciences, 5(2), 353–369. https://doi.org/10.5194/bg-5-353-2008

Bourdin, G., Karp-Boss, L. (2023) Pigment concentrations derived from High-Performance Liquid Chromatography (HPLC) analysis from samples collected during the Tara Pacific expedition from 2016-2018. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2023-02-24 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.889930.1 [access date]

Terms of Use

This dataset is licensed under Creative Commons Attribution 4.0.


If you wish to use this dataset, it is highly recommended that you contact the original principal investigators (PI). Should the relevant PI be unavailable, please contact BCO-DMO (info@bco-dmo.org) for additional guidance. For general guidance please see the BCO-DMO Terms of Use document.

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Funded by the U.S. National Science Foundation