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Dataset: DOC uptake rates by coccolithophores and scintillation counts from field experiments in the North Atlantic during R/V Endeavor cruise EN616 in July 2018
Final no updates expectedDOI: 10.26008/1912/bco-dmo.887562.1Version 1 (2023-03-22)Dataset Type:Cruise ResultsDataset Type:experimental
Field experiments were performed in the NW Atlantic during R/V Endeavor cruise EN616 in July 2018 to determine the potential uptake of dissolved organic carbon (DOC) by coccolithophores to compare with the bicarbonate uptake associated with standard photosynthesis and calcification. Waters from the continental shelf and slope, plus Sargasso Sea were sampled. Natural phytoplankton populations were incubated with radiolabeled dissolved organic carbon compounds (14C-acetate, 14C-mannitol, and 14C-...
Statistics of 10 count averages for incubation experiments
Parameters for the file are described below:
Parameter, Description, Units, Data_type,,
Start_Time_UTC,Start datetime of incubation experiment (time of inoculation) in UTC with ISO8601 format,unitless,Datetime,%Y-%m-%dT%H:%M:%SZ
End_Time_UTC,End datetime of incubation experiment (time of filtration or sorting) in UTC with ISO8601 format,unitless,Datetime,%Y-%m-%dT%H:%M:%SZ
Latitude, Latitude of incubation station, decimal degrees, Float,,
Longitude, Longitude of incubation station, decimal degrees, Float,,
Experiment_ID, Experiment ID, unitless, String,,
Sample_num, Sample number, unitless, Integer,,
Compound, "Organic compound being measured (acetate, mannitol, glycerol, bicarbonate)", unitless, String,,
Incubation, Incubation number where 1 to 3 are replicates and 4 is formalin-treated incubation, unitless, Integer,,
Type, Type of sample (bulk or flow cytometer sorted), unitless, String,,
SampleID, Sample ID, unitless, String,,
Average_10ct, Ten-count average of the disintegrations per minute (DPM) , DPM, Float,,
StdDev_10ct, Standard deviation of the ten-count average, DPM, Float,,
CI_95pct_10ct,95% Confidence Interval for ten-count average, DPM, Float,,
Sample_DPM_minus_blank_DPM, Ten-count average minus the formalin blank, DPM, Float,,
StdDev_sample_minus_blank, "Standard deviation of the 'sample minus blank' values, for ten-count average and calculated using standard error propagation", DPM, Float,,
Incub_reps_avg, Average of three replicate incubations with no blank subtracted, DPM, Float,,
StdDev_incub_3reps, Standard deviation of the three replicate incubations, DPM, Float,,
CI_95pct_3reps,95% Confidence Interval for replicate counts based on the standard deviation of replicate incubations and three x 10-replicate counts within each experiment, DPM, Float,,
Coeff_Var, Coefficient of Variation, Percent, Float,,
Calcification,Indicator for calcification process; Entries with no C indicate incorporation into organic matter,unitless,String,
Comments,"Comments about sample, experiment, or other relevant information",unitless,String,
Start_Time_EDT,Start datetime of incubation experiment (time of inoculation) in EDT time zone,unitless,Datetime,%Y-%m-%d %H:%M:%S
End_Time_EDT,End datetime of incubation experiment (time of filtration or sorting) in EDT time zone,unitless,Datetime,%Y-%m-%d %H:%M:%S
This dataset is part of a larger study with the following goals:
Goal #1: measure the mixotrophic uptake and assimilation of 14C-acetate, 14C-mannitol and 14C-glycerol as a carbon source by natural assemblages of coccolithophores and compare it to their autotrophic uptake and assimilation of DIC. These three organics were chosen due to their high potential for significant osmotrophy by coccolithophores as seen in previous culture studies (Godrijan et al. (2020) and related dataset: https://www.bco-dmo.org/dataset/858771). The design of these experiments used radiochemical and single cell/flow cytometer methods to distinguish osmotrophy by coccolithophores from that of other naturally-occurring microalgae.
Goal #2: test for the fixation of 14C-labeled organics into both POC and PIC fractions in natural populations of coccolithophores, in order to examine the potential role of coccolithophore osmotrophy in the biological carbon pump and alkalinity carbon pump paradigms.
Balch, W. M., Archer, S. D., Drapeau, D. T., Godrijan, J. (2023) Coccolithophore counts from polarized microscopy birefringence measurements of samples collected in the Northwest Atlantic during R/V Endeavor cruise EN616 in July 2018. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2023-02-05 doi:10.26008/1912/bco-dmo.887863.1
Balch, W. M., Archer, S. D., Drapeau, D. T., Godrijan, J. (2023) FlowCAM enumeration of phytoplankton classes from samples taken during R/V Endeavor cruise EN616 in July 2018. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2023-01-27 doi:10.26008/1912/bco-dmo.887787.1
Balch, W. M., Archer, S. D., Drapeau, D. T., Godrijan, J. (2023) Ambient concentrations of acetate, glycerol, and mannitol measured from samples collected during R/V Endeavor cruise EN616 in the northwest Atlantic in July 2018. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2023-02-26 doi:10.26008/1912/bco-dmo.887851.1
Balch, W. M., Archer, S. D., Drapeau, D. T., Godrijan, J. (2023) Hydrography and environmental conditions measured with CTD at nine stations during R/V Endeavor cruise EN616 in July 2018. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2023-02-24 doi:10.26008/1912/bco-dmo.887800.1
Related Publications
Results
Balch, W. M., Drapeau, D. T., Poulton, N., Archer, S. D., Cartisano, C., Burnell, C., & Godrijan, J. (2023). Osmotrophy of dissolved organic compounds by coccolithophore populations: Fixation into particulate organic and inorganic carbon. Science Advances, 9(21). https://doi.org/10.1126/sciadv.adf6973
Methods
Balch, W. M., Drapeau, D. T., & Fritz, J. J. (2000). Monsoonal forcing of calcification in the Arabian Sea. Deep Sea Research Part II: Topical Studies in Oceanography, 47(7–8), 1301–1337. https://doi.org/10.1016/s0967-0645(99)00145-9
Methods
Paasche, E., & Brubak, S. (1994). Enhanced calcification in the coccolithophorid Emiliania huxleyi (Haptophyceae) under phosphorus limitation. Phycologia, 33(5), 324–330. https://doi.org/10.2216/i0031-8884-33-5-324.1
Related Research
Godrijan, J., Drapeau, D., & Balch, W. M. (2020). Mixotrophic uptake of organic compounds by coccolithophores. Limnology and Oceanography, 65(6), 1410–1421. doi:10.1002/lno.11396
Related Research
Hewes, C. D., & Holm-Hansen, O. (1983). A method for recovering nanoplankton from filters for identification with the microscope: The filter-transfer-freeze (FTF) technique. Limnology and Oceanography, 28(2), 389–394. Portico. https://doi.org/10.4319/lo.1983.28.2.0389
Balch, W. M., Godrijan, J., Drapeau, D. T., Archer, S. D. (2023) DOC uptake rates by coccolithophores and scintillation counts from field experiments in the North Atlantic during R/V Endeavor cruise EN616 in July 2018. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2023-03-22 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.887562.1 [access date]
If you wish to use this dataset, it is highly recommended that you contact the original principal investigators (PI). Should the relevant PI be unavailable, please contact BCO-DMO (info@bco-dmo.org) for additional guidance. For general guidance please see the BCO-DMO Terms of Use document.