©2020 Biological and Chemical Oceanography Data Management Office.Chloroplast 23S genotypes (based on length heteroplasmy in domain V of chloroplast large subunit (cp23S) ribosomal DNA sequences) of Breviolum sp. symbionts within Orbicella faveolata recruits that were outplanted to reefs in the Florida Keys.
Sampling and Analytical Methodology:
Egg-sperm bundles were collected from adult colonies of O. faveolata following the techniques described by Miller (2014). Larvae were reared at a shore-based lab and settled onto terracotta tiles. Once the larvae had metamorphosed and attached to the tiles, they were outplanted onto the reef by attachment vertically to a PVC rack approximately 0.2 m above the substrate to allow uptake of algal symbionts. Tiles with recruits were sampled one to three months after outplanting, recruits removed from the tile and preserved individually in 95% ethanol for subsequent molecular analysis. DNA extraction followed Coffroth et al. (1992). Cp-23S genotypes of the algal symbionts within the genus Breviolum were characterized following the protocol of Santos et al. (2003).
Locations in the Florida Keys with Abbreviations
Coffroth, M. A., Miller, M. W., Sheets, D. (2023) Breviolum symbiont CP23S genotypes in Orbicella faveolata recruits from 2009-2011 (SymBioSys project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2023-01-24 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.882097.1 [access date]
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