©2020 Biological and Chemical Oceanography Data Management Office.This dataset represents the global proteome of replete laboratory cultures of Alteromonas macleodii MIT1002 (collected in triplicate). This dataset is an initial examination of the proteome allocation of this heterotrophic bacteria and will contribute to C-CoMP's efforts that are focused on understanding the physiology of model marine bacteria. A total of 2075 proteins were identified in MIT1002. The Moran laboratory at University of Georgia grew and prepared the cultures and the Saito laborator...
Show moreCultures (50 ml each) of Ruergeria pomeroyi DSS-3 and Alteromonas macleodii MIT1002 were harvested in triplicate, centrifuged at 8000 x g for 10 minutes, flash frozen, stored at -80 degrees C, and shipped to Woods Hole Oceanographic Institution for proteomics analysis. Cultures were filtered and filters were extracted using SDS detergent and a SP3 magnetic bead purification followed by trypsin digestion alkylation and reduction. The methods are described in Saito et al. 2020 and were modified from the methods originally published by Hughes et al. 2014. Analysis was conducted by 2D active modulation Orbitrap mass spectrometry as described in McIlvin and Saito 2021.
This dataset reports results for the Alteromonas macleodii MIT1002 samples. See related datasets for the Ruergeria pomeroyi DSS-3 samples.
Note: the sample ids ending in a1, b1, and c1 differentiate biological triplicates from one another.
Saito, M. A., Moran, M. A. (2022) The global proteome of replete laboratory cultures of Alteromonas macleodii MIT1002. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2022-06-14 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.875612.1 [access date]
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