©2020 Biological and Chemical Oceanography Data Management Office.Experimental calcification results from crustose coralline algae experiment involving multiple temperatures and pCO2 levels. Clathromorphum compactum and C. nereostratum were used for this experiment.
Methodology:
Sampling and analytical procedures:
C. compactum and C. nereostratum cultured for 4 months under 12 pCO2/T treatments in 42 liter aquaria.
Seawater samples were obtained every ~14 days using 250 mL ground-glass-stoppered borosilicate glass bottles for measurement of total alkalinity (TA) and dissolved inorganic carbon (DIC) and other carbonate parameters.
Temp, salinity, pH were measured three times weekly.
For buoyant weight, specimens were suspended beneath a balance in an aquarium at 4 cm depth in seawater of constant temperature and salinity.
Specimens were dosed with calcein before the experiment and the vertical growth was determined by measuring the vertical growth from the resulting calcein line to the surface.
Westfield, I., Ries, J. B., Williams, B., Rasher, D. B. (2022) Clathromorphum compactum and C. nereostratum calcification experiment data involving multiple temperatures and pCO2 levels (CorallineAlgaePaleo-pH). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2022-04-04 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.871633.1 [access date]
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