©2020 Biological and Chemical Oceanography Data Management Office.This dataset includes phytoplankton diagnostic pigments from HPLC from samples collected on R/V Endeavor cruise EN640 in the tropical North Atlantic during June-July 2019. These data are also available through NASA's SeaBASS repository.
The samples were collected using a CTD rosette and an appropriate volume of water was filtered through a 25mm GF/F filter soon after collection. The filters were then stored in liquid nitrogen until analyzed.
The diagnostic pigments were measured using High-performance liquid chromatography as detailed in Van Heukelem and Thomas (2001) and Hooker et al (2005).
The HPLC used for pigment analysis is an Agilent RR1200 with a programmable autoinjector (900 ul syringe head), refrigerated autosampler, degasser, and photo-diode array detector with deuterium and tungsten lamps. The HPLC is controlled by Agilent Chemstation software. The 4.6 x 150 mm HPLC Eclipse XDB column (Agilent Technologies, Palo Alto, CA) is filled with a C8 stationary phase (3.5 um stationary phase); the mobile phase consists of a linear gradient from 5-95% solvent B over the 27 minutes, for which solvent A is 70 parts methanol, 30 parts 28 mM tetrabutylammononium acetate (pH 6.5) and solvent B is methanol. The column temperature is 60 C and the photo diode array detector is set to plot chromatograms at 450, 665, and 222 nm to acquire visible absorbance spectra between 350 and 750 nm.
Subramaniam, A. (2021) Phytoplankton diagnostic pigments from HPLC from samples collected on R/V Endeavor cruise EN640 in the tropical North Atlantic during June-July 2019. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2021-12-08 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/866351 [access date]
Terms of Use
This dataset is licensed under Creative Commons Attribution 4.0.
If you wish to use this dataset, it is highly recommended that you contact the original principal investigators (PI). Should the relevant PI be unavailable, please contact BCO-DMO (info@bco-dmo.org) for additional guidance. For general guidance please see the BCO-DMO Terms of Use document.