File(s) | Type | Description | Action |
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t_rotula_microbiome_global.csv (12.58 KB) | Comma Separated Values (.csv) | Primary data file for dataset ID 860347 | Add to Cart Download |
This dataset includes NCBI identifiers and descriptions of T. rotula microbiome global samples.
Single cells and chains of T. rotula were subjected to three serial washes in separate aliquots of 200 μl sterile seawater, with <2 μl seawater carried between washes. Individual cells or chains were then cultured with associated bacteria in 1ml f/20 in sterile polystyrene plates at ~in situ temperature and 90-120 μmol photons m⁻² s⁻¹ on a 12:12-h light:dark cycle. Isolates were grown for two weeks, or until the phytoplankton concentration reached ~1,000 cells mL⁻¹, whichever occurred sooner. Single cell isolates were filtered onto 0.2-μm filters (Millipore) and stored at -80 °C until DNA extraction. DNA was extracted from T. rotula isolates and bacterial co-cultures following Whittaker and Rynearson (2017).
Rynearson, T. A. (2021) T. rotula microbiome global sample. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2021-09-13 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.860347.1 [access date]
Terms of Use
This dataset is licensed under Creative Commons Attribution 4.0.
If you wish to use this dataset, it is highly recommended that you contact the original principal investigators (PI). Should the relevant PI be unavailable, please contact BCO-DMO (info@bco-dmo.org) for additional guidance. For general guidance please see the BCO-DMO Terms of Use document.