Particulate organic phosphorus data from TriCoLim incubation experiments done aboard R/V Atlantis AT39-05 in the Tropical Atlantic
Shipboard incubation experiments were carried out at 15 stations in the tropical Atlantic with trace metal clean-collected bulk seawater to examine iron (Fe) and phosphorus (P) co-limitation of dinitrogen (N2) fixation (see TriCoLim Station coordinates metadata). Treatments were: Co-limited control (no additions, “Colimit”), + 2nM Fe (“Fe”), + 50 nM P (“P”) and Replete (+Fe+P, “Rep”). All incubations were carried out at ambient (“Lo”) temperature and ambient temperature +3C (“Hi”) for 2-4 days on deck before measuring 15N2 and 13CO2 fixation rates and particulate organic carbon (POC), nitrogen (PON) and phosphorus (POP). See related datasets.
POP measurements were conducted as follows: ~500 ml of bulk water were filtered onto pre-combusted GFF filters and rinsed two times with 2 ml each of 0.17M sodium sulfate (Na2SO4). Filters were placed in pre-combusted borosilicate vials capped a combusted aluminum foil cap and soaked in 2 ml of 0.017M magnesium sulfate (MgSO4) and left to dry in an oven at 60 deg C for 2-3 days.
Hutchins, D. A., Fu, F. (2021) Particulate organic phosphorus data from TriCoLim incubation experiments done aboard R/V Atlantis AT39-05 in the Tropical Atlantic. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2021-07-13 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/855661 [access date]
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