©2020 Biological and Chemical Oceanography Data Management Office.This dataset contains results from genus-specific qPCR assays to quantify the abundance of Symbiodinium, Breviolum, Cladocopium, and Durusdinium symbionts relative to coral cells in Orbicella faveolata from Abaco, The Bahamas in January 2019. Bulk genomic DNA was extracted from tissue scrapings collected by SCUBA divers, and used as a template for qPCR assays. Resulting CT values were used to calculate symbiont to host cell ratios for each symbiont genus within each coral. The publication based ...
Show moreTissue samples from colonies of O. faveolata were collected from Mermaid Reef and sensitive Sandy Cay Reef between 21 and 24 January 2019. Genomic DNA from each coral sample was extracted and assayed using qPCR targeting loci specific to O. faveolata and each of four symbiont genera (Symbiodinium, Breviolum, Cladocopium, and Durusdinium). All reactions were performed on a QuantStudio 3 Real-Time PCR system (Life Technologies, Foster City, CA, USA). The resulting cycle threshold (CT) values at a set threshold of delta Rn = 0.01 were used for data analysis.
Parker, K., Cunning, R. (2021) Symbiont community structure in Orbicella faveolata from Mermaid Reef and Sandy Cay Reef in Abaco, The Bahamas in January 2019. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2021-11-12 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.855439.1 [access date]
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