File(s) | Type | Description | Action |
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chl_a.csv (2.32 KB) | Comma Separated Values (.csv) | Primary data file for dataset ID 848948 | Add to Cart Download |
This dataset reports the size-fractionated chlorophyll a from multivariate mesocosm experiments conducted with a natural phytoplankton community from Narragansett Bay, RI. These data were assessed in Anderson et al. The Interactive Effects of Temperature and Nutrients on a Spring Phytoplankton Community (in prep).
Multivariate mesocosm experiments were conducted with a natural phytoplankton community from Narragansett Bay, RI. Water was incubated in triplicate at -0.5ºC, 2.6ºC, and 6ºC for 10 days. At each temperature, treatments included both nutrient amendments (N, P, Si addition) and controls (no macronutrients added). For complete methodology, see “The Interactive Effects of Temperature and Nutrients on a Spring Phytoplankton Community” (Anderson et al, in prep).
Community size structure from incubation experiments was assessed using size-fractionated chlorophyll a. At each dilution time point, whole incubation water was filtered in triplicate onto 25 mm Whatman GF/F filters (GE Healthcare), 5 µm polyester filters, or pre-filtered through a 20 µm mesh and then filtered onto 25 mm GF/F filters. This allowed for the calculation of the following size fractions: 0.7-5 µm, 5-20 µm, and >20 µm. Chlorophyll was then extracted in 90% acetone for 24 hours at -20ºC. Fluorescence was read on a 10-AU fluorometer (Turner Designs) and data was analyzed according to the techniques described in Graff and Rynearson (2011).
Anderson, S. I., Franze, G., Kling, J. D., Wilburn, P., Kremer, C. T., Menden-Deuer, S., Litchman, E., Hutchins, D. A., Rynearson, T. A. (2021) Size-fractionated chlorophyll a from multivariate mesocosm experiments conducted with a natural phytoplankton community from Narragansett Bay, RI. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2021-04-14 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.848948.1 [access date]
Terms of Use
This dataset is licensed under Creative Commons Attribution 4.0.
If you wish to use this dataset, it is highly recommended that you contact the original principal investigators (PI). Should the relevant PI be unavailable, please contact BCO-DMO (info@bco-dmo.org) for additional guidance. For general guidance please see the BCO-DMO Terms of Use document.