©2020 Biological and Chemical Oceanography Data Management Office.Fluorometer data (volts) from CTD casts from NOAA Ship R/V Nancy Foster cruises NF1704 and NF1802 in the Gulf of Mexico, May 2017 and May 2018.
Samples for HPLC chlorophyll were collected pre-dawn from Niskin bottles mounted on a 24-place rosette system equipped with a Seabird SBE911 CTD and a Seapoint fluorometer. Seapoint fluorometer data (volts) for chlorophyll a is compared with discrete HPLC bottle data.
HPLC samples (2.2-L) were collected for pigment analyses by high-pressure liquid chromatography (HPLC) at the Horn Point Analytical Services Laboratory (University of Maryland Center for Environmental Science). Pigment samples (2.2-L) were filtered onto GF/F filters, frozen in LN2 and stored at -85°C until extracted, and analyzed using an automated 1100 HPLC system with Agilent temperature-controlled autosampler, Peltier temperature-controlled column oven compartment, PDA detector and ChemStation software. The HPLC method uses a C8 column and a reversed phase, methanol-based solvent system (Van Heukelem and Thomas, 2001; Hooker et al., 2012). MVCHLa and DVCHLa are detected at 665 nm. Carotenoid and xanthophyll accessory pigments are detected at 450 nm.
Selph, K. E. (2021) Fluorometer data (volts) from CTD casts from NOAA Ship R/V Nancy Foster cruises NF1704 and NF1802 in the Gulf of Mexico, May 2017 and 2018. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2021-01-07 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.835566.1 [access date]
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