©2020 Biological and Chemical Oceanography Data Management Office.Results of the quantification of symbiont cell numbers from 400 Acropora hyacinthus colonies subjected to experimental bleaching in the summer of 2018 in Palau.
These samples were collected according to the protocol published by Krediet et al. 2015: Article Rapid, Precise, and Accurate Counts of Symbiodinium Cells Using the Guava Flow Cytometer, and a Comparison to Other Methods
The samples that were analyzed for this study are 400 colonies of Acropora hyacinthus. Nubbins from each colony were experimentally bleached at temperatures of 34, 34.5 and 35 degrees Celsius as well as two control treatments (30 deg. C). These nubbins were then airbrushed and the resulting tissue slurry was preserved in RNAlater. Tissue dissociation and preparation for analysis on the Guava EasyCyte flow cytometer followed the protocol by Krediet et al. 2015.
Procedures followed Krediet et al. 2015 (above), with the exception that protein concentration was not measured.
Palumbi, S. (2020) Results of the quantification of symbiont cell numbers from 400 Acropora hyacinthus colonies subjected to experimental bleaching in the summer of 2018 in Palau.. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2020-06-23 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.813210.1 [access date]
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