Dataset: Microbial abundance of phytoplankton and bacteria from RV/Tangaroa cruise TAN1810SALP to Chatham Rise vicinity, east of New Zealand, Oct - Nov. 2018

Sampling and analytical procedures: Samples (15 mL) were collected from hydrographic casts with Niskin bottles on a CTD-rosette for live ship-board and preserved shore-based flow cytometry analyses for profiles of phytoplankton and heterotrophic (non-pigmented) bacteria abundance.  Live samples (~800 uL) were analyzed within 2 hours of collection (and usually sooner), kept in the dark at room temperature prior to analysis.  Samples (2 mL) for shore-based analyses were aliquoted into cryovials, preserved with a final concentration of 0.5% paraformaldehyde (Electron Microscopy Sciences, methanol-free), then flash frozen in liquid nitrogen.  After freezing, samples were transferred to a -80°C freezer.  Upon completion of the cruise, samples were transferred to a charged dry shipper for transport to the University of Hawaii.  Samples were thawed in batches, stained with the DNA stain Hoechst 34580 for 1 hour (1 ug/ml, final concentration), then quantitatively analyzed (100 uL).

Offline analysis software: FlowJo Software (Mac) Version 10.6.1. Ashland, OR: Becton Dickinson and Company; 2019.Offline analysis software: FlowJo Software (Mac) Version 10.6.1. Ashland, OR: Becton Dickinson and Company; 2019.