Dataset: 16S rRNA gene amplicon sequences from cathodic poised potential experiments with subsurface crustal samples from CORK borehole observatories at North Pond on the Mid-Atlantic Ridge during R/V Atlantis cruise AT39-01

Release Date:2020-02-01Final no updates expectedVersion 1 (2019-10-30)Dataset Type:Cruise ResultsDataset Type:experimental

Principal Investigator: Beth N. Orcutt (Bigelow Laboratory for Ocean Sciences)

Co-Principal Investigator: Timothy D'Angelo (Bigelow Laboratory for Ocean Sciences)

BCO-DMO Data Manager: Amber D. York (Woods Hole Oceanographic Institution)

Program: Center for Dark Energy Biosphere Investigations (C-DEBI)

Project: Collaborative Research: Completing North Pond Borehole Experiments to Elucidate the Hydrology of Young, Slow-Spread Crust (North Pond 2017)

Abstract

16S rRNA gene amplicon sequences from cathodic poised potential experiments with subsurface crustal samples from CORK borehole observatories at North Pond on the Mid-Atlantic Ridge during R/V Atlantis cruise AT39-01. Illumina amplicon sequence data (V4-V5 region of the 16S rRNA gene) is publicly available through NCBI Sequence Read Archive under BioProject PRJNA564565 samples SAMN12723345-SAMN12723415. BioProject PRJNA564565: https://www.ncbi.nlm.nih.gov/bioproject/PRJNA564565

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16S rRNA gene amplicon sequences from cathodic poised potential experiments with subsurface crustal samples from CORK borehole observatories at North Pond on the Mid-Atlantic Ridge during R/V Atlantis cruise AT39-01.

Illumina amplicon sequence data (V4-V5 region of the 16S rRNA gene) is publicly available through NCBI Sequence Read Archive under BioProject PRJNA564565 samples SAMN12723345-SAMN12723415. 
https://www.ncbi.nlm.nih.gov/bioproject/PRJNA564565

These results were published in Jones et al. (2020).

Related datasets from the same experiment:
AT39-01 CathodicEET Chronoamperometry: https://www.bco-dmo.org/dataset/780127
AT39-01 CathodicEET CyclicVoltametry: https://www.bco-dmo.org/dataset/780248
AT39-01 CathodicEET SEM: https://www.bco-dmo.org/dataset/780261
AT39-01 CathodicEET Experimental Metadata:
https://www.bco-dmo.org/dataset/780225

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Related Publications

Results
Jones, R. M., D’Angelo, T., & Orcutt, B. N. (2020). Using Cathodic Poised Potential Experiments to Investigate Extracellular Electron Transport in the Crustal Deep Biosphere of North Pond, Mid-Atlantic Ridge. Frontiers in Environmental Science, 8. doi:10.3389/fenvs.2020.00011
Methods
DAngelo, T. (2018). DNA Extraction using FastDNA Spinkit for Soil with PolyA Modifictaion v1 (protocols.io.ur8ev9w). Protocols.io. doi:10.17504/protocols.io.ur8ev9w

Orcutt, B. N., D'Angelo, T. (2019) 16S rRNA gene amplicon sequences from cathodic poised potential experiments with subsurface crustal samples from CORK borehole observatories at North Pond on the Mid-Atlantic Ridge during R/V Atlantis cruise AT39-01. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2019-10-30 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/780255 [access date]

Terms of Use

This dataset is licensed under Creative Commons Attribution 4.0.


If you wish to use this dataset, it is highly recommended that you contact the original principal investigators (PI). Should the relevant PI be unavailable, please contact BCO-DMO (info@bco-dmo.org) for additional guidance. For general guidance please see the BCO-DMO Terms of Use document.

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Funded by the U.S. National Science Foundation