These data were published in Comeau et al. (2017).
See Comeau et al. (2017) for a detailed overview of the methodology.
Locations:
UCB Gump Research Station Moorea, French Polynesia
Hawaii Institute of Marine Biology, Kaneohe Bay, O’ahu, HI
Methodology:
For the three experiments, pH on the total scale (pHT) was measured daily using a portable pH meter (Orion 3-stars, Thermo-Scientific, USA) fitted with a DG 115-SC pH probe (Mettler Toledo, Switzerland) calibrated every other day with Tris/HCl buffers (Dickson et al., 2007). pHT also was mea- sured every 2 weeks spectrophotometrically using m-cresol dye (Dickson et al., 2007). Mean values of pHT measured spectrophotometrically and using a pH electrode differed by < 0.02 pH units. Total alkalinity (AT ) was measured using open-cell potentiometric titrations (Dickson et al., 2007) on ∼ 50 g samples of seawater collected every 2–3 days. Parameters of the carbonate system in seawater were determined with the seacarb R package (Gattuso et al., 2015) using measured values of pHT , AT , temperature, and salinity.
Net community calcification (Gnet) in the flumes was mea- sured using the total alkalinity anomaly method (Chisholm and Gattuso 1991), and net community primary production (Pnet) was measured using oxygen sensors (TROLL 9500, In-Situ) that measured the O2 concentration at 60 s intervals with an accuracy of 0.2 mg L−1.
Carpenter, R., Edmunds, P. J., Comeau, S. (2019) Comeau 2017 Biogeosciences: calcification and chemistry. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2019-02-01 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/754750 [access date]
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