File(s) | Type | Description | Action |
---|---|---|---|
SPOT_cruise.csv (12.20 KB) | Comma Separated Values (.csv) | Primary data file for dataset ID 718580 | Add to Cart Download |
Location: San Pedro Ocean Time Series (SPOT) station (33°33′N, 118°24′W)
Samples for quantification of retinal oxime were collected at a six of depths within the euphotic zone (5-250m). Seawater was collected from each CTD depth using Niskin bottles and immediately filtered. Particulate samples were collected using in-line 0.2µm, 3µm and 10µm pore-size filters and a peristaltic pump (flow rate < 50 ml per minute), transferred into sterile cryovials and were immediately stored at -80 degrees C until analysis.
Pigments were extracted from the filters in 3 mL of methanol, BHT (butylated hydroxytoluene) was added and placed in a -20 degrees C freezer overnight. The retinal oxime was formed by the addition of hydroxylamine hydrochloride and irradiated under yellow light for 2 hours before analysis. Retinal oxime samples were analyzed by liquid chromatography/triple mass spectrometry (LC/MS/MS/MS). The LCMS system consists of a ThermoTSQ Quantum Access electro-spray ionization triple quadrupole mass spectrometer, coupled to a Thermo Accela High Speed Liquid Chromatography system.
For chlorophyll-a measurements, 100 microliters of the pigment extraction were diluted in acetone (50x dilution) and analyzed using a Turner 10AU fluorometer.
Bacterial production was estimated by incorporation of [3H] thymidine and [3H] leucine into DNA and protein, respectively, as earlier described (Simon & Azam, 1989, Fuhrman and Azam 1982).
Sanudo-Wilhelmy, S. A., Fuhrman, J. A. (2017) Retinoid data from SPOT cruises during different months (2016-2017). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2017-11-03 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/718580 [access date]
Terms of Use
This dataset is licensed under Creative Commons Attribution 4.0.
If you wish to use this dataset, it is highly recommended that you contact the original principal investigators (PI). Should the relevant PI be unavailable, please contact BCO-DMO (info@bco-dmo.org) for additional guidance. For general guidance please see the BCO-DMO Terms of Use document.