©2020 Biological and Chemical Oceanography Data Management Office.Measurements of peptidase and glucosidase activities in large volume mesocosm incubations on RV/Endeavor EN584, July 2016 (Patterns of activities project)
Fluorescence was measured over 24-48 hours incubation time with a plate reader (TECAN spectrafluor plus; 360 nm excitation, 460 emission), with time points taken every 4-6 hours. Hydrolysis rates were calculated from the rate of increase of fluorescence in the incubation over time relative to a set of standards of known concentration of fluorophore. Scripts to calculate hydrolysis rates and produce the figures shown here are available in the associated Github repository [Hoarfrost, 2017].
See Niskin Bottle and Cast List EN584 to link specific casts and bottles to each experiment: BCO-DMO dataset 717427 (see related datasets).
Arnosti, C. (2022) Measurements of peptidase and glucosidase activities in large volume mesocosm incubations on RV/Endeavor EN584, July 2016 (Patterns of activities project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2022-12-07 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.717532.1 [access date]
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