©2020 Biological and Chemical Oceanography Data Management Office.Somatic mutations that occurred at 5 microsatellite loci in Acropora palmata collected throughout the Caribbean. These data were deposited in DRYAD: http://dx.doi.org/10.5061/dryad.f6600 and published in the paper Devlin-Durante et al, Mol Ecol. (2016).
Adult Acropora palmata colonies were sampled (1 cm2) with hammer and chisel and tissues were preserved in 96% non-denature Ethanol. DNA was extracted using the DNEasy tissue kit (Qiagen) following manufacturer’s instructions. Two multiplex Polymerase Chain Reactions (PCR) were performed per sample using fluorescently labeled primers to assay five loci containing AAT repeats. These five microsatellite loci have previously been demonstrated to be mendelian and coral-specific using controlled crosses (Baums et al., 2005). PCR products were visualized with an automated sequencer (ABI 3730). An internal size standard (Gene Scan 500-Liz, Applied Biosystems CA) ensured accurate sizing. Electropherograms were analyzed with GeneMapper Software 3.0 (Applied Biosystems, CA). Alleles were scored as PCR product size.
Baums, I. B. (2021) Mutations at microsatellite loci of Acropora palmata in the Caribbean and North-West Atlantic from 2015-2016 (Coral Hybridization project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 2) Version Date 2017-10-19 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.666321.2 [access date]
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