Dataset: Data from various stages of the analysis pipeline on coral collected sampled in the Caribbean during 2013 (Contagious coral diseases project)

This dataset contains analysis pipeline files. There are 3 types of files:

1. One set has the extension “.fa”. These are the fasta files in various stages through our analysis pipeline.  

2. Additionally, several chimera checks are performed. Chimera sequences are placed in chimera files e.g.  "fasta.chimeras.fa."

3. One file contains a cluster for error correction and output consensus "cons.fa"

Coral disease transmission experiments were completed for dark-spot syndrome on Sidereastrea siderea and yellow-band disease on Orbicella faveolata, as described in Randall et al. 2016. Following experimentation, microbial communities were extracted from tissue samples to determine whether any potential pathogen may have transmitted from healthy to exposed corals. Microbial communities on healthy corals were compared with diseased corals to identify any potential pathogens.

Experimental diseased and healthy corals were sampled and their microbial communities were analyzed using 454 Illumina pyrosequencing of the amplified 16S rRNA gene on the V1-V3 hypervariable region.