©2020 Biological and Chemical Oceanography Data Management Office.Prochlorococcus MED4 was acclimated to low (150 ppm) or high (1000 ppm) CO2. The cultures were then concentrated and placed in the chamber of a membrane inlet mass spectrometer (MIMS; Pfieffer QMS220). Dissolved inorganic carbon (DIC) was gradually added to the culture and inorganic carbon fluxes were measured using MIMS as described in Hopkinson et al. 2014.
Relevant Reference:
Hopkinson, B.M., J.N. Young, A. L. Tansik, and B. J. Binder. 2014. The minimal CO2-concentrating mechanism of Prochlorococcus spp. MED4 is effective and efficient. Plant Physiology 166: 2205-2217.
Hopkinson, B. M. (2015) Experimental results on inorganic carbon fluxes of Prochlorococcus MED4 cultured under high and low CO2 concentrations (OA phytoplankton physiology project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 2015-07-29) Version Date 2015-07-29 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/563152 [access date]
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