©2020 Biological and Chemical Oceanography Data Management Office.An approach was developed to recover prey DNA from gut contents of nauplii of a predatory copepod by blocking the detection of predator, Tortanus dextrilobatus, with ligase and a blocking oligonucleotide and amplifying prey DNA with universal primers. Adding 25 U of ligase improved predator clamping, while 100 and 200 U reduced efficiency. Field collected nauplii consumed a broad range of prey.This dataset measures the performance of the blocking oligonucleotide with increasing amounts of ligase and at two ratios of prey to predator DNA concentration. Data are also shown for wild nauplii at 25 and 100 U of ligase.
This dataset is published in Figure 2, Craig et al (2014).
Related Reference:
* Craig, Carrie, Wim J. Kimmerer, and C. Sarah Cohen. 2014. A DNA-based method for investigating feeding by copepod nauplii. Journal of Plankton Research 36 (1): 271-275
Vogt, R.A., T.R. Ignoffo, L.J. Sullivan, J. Herndon, J.H. Stillman, and W. Kimmerer. 2013. Feeding capabilities and limitations in the nauplii of two pelagic estuarine copepods, Pseudodiaptomus marinus and Oithona davisae. Limnology and Oceanography 58: 2145-2157.
Kimmerer, W., Cohen, S. (2015) Experimental results describing Copepod fed phytoplankton mixtures that were analyzed at San Francisco State University in 2013. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 2015-01-15) Version Date 2015-01-15 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/546536 [access date]
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